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KMID : 0861020120270060055
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Korea Journal of Herbology
2012 Volume.27 No. 6 p.55 ~ p.61
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The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea
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Bae Gi-Choon
Bae Dae-Yeoll
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Abstract
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Objectives: Allium Hookeri (AH) is a traditional herb to treat inflammatory diseases in India and Myanmar. Recently, AH cultivation was succeeded in South Korea. This study was performed to evaluate the anti-inflammatory effects of Korean AH in RAW264.7 cells, mouse macrophage cell line.
Methods: To evaluate the anti-inflammatory effects of root of AH, we prepared the 70% ethanol extract, then we examined the productions of nitrite, and pro-inflammatory cytokines. To examine the nitrite, and cytokines, the RAW264.7 cells were treated with AH, then stimulated with lipopolysaccharide (LPS, 500 ng/ml) for 24 h. Then the cells were harvested for griess assay, ELISA and real-time reverse transcription polymerase chain reaction (RT-PCR). Also to detect the ability of AH to induce heme oxygenase-1 (HO-1), we examined the HO-1 expression using real time RT-PCR and western blot. Furthermore, we examined the mitogen activated-protein kinases (MAPKs) and nuclear factor kappa B (NF-ĸB) activation to find out the underlying mechanisms.
Results: AH ethanol extract significantly inhibited the productions of nitrite and interleukin (IL)-1β. AH treatment increased the HO-1 expression dramatically at 1 h, then peaked at 3 h. When the HO-1 was inhibited by tin (Sn) protoporphryin-IX (SnPP), the anti-inflammatory action of AH was reversed. AH treatment inhibited the activation of p38, but not extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of inhibitory kappa B a (Ik-Bα) in the LPS-stimulated RAW 264.7 cells.
Conclusions: These data could suggest that AH exerts anti-inflammatory influences through up-regulation of HO-1 and deactivation of p38.
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KEYWORD
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Allium Hookeri (AH), lipopolysaccharide (LPS), macrophages, inflammation, heme oxygenase-1 (HO-1)
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